ผลงานวิจัยปี 2562

No.

Title, author, link, abstract

Journal

Development of a portable screen-printed graphene electrode for determination total gold ions in environments

Phichanan Duchda, Weena Siangproh

http://ijaseat.iraj.in/paper_detail.php?paper_id=15671&name=Development_of_a_Portable_Screen-Printed_Graphene_Electrode_for_Determination_Total_Gold_Ions_in_Environments

Abstract

A new electrochemical sensor using paper-based screen-printed graphene electrode (SPGE) was first developed here for the determination of gold ions in environmental waste water using square wave voltammetry. The procedure based on the reduction of gold ions at graphene electrode surface. The effects of basic experimental parameters on the response of the SPGE electrode were investigated to obtain the optimal operating conditions. Under the optimal conditions, a wide linear range of 1 - 200 mg×L-1 (y = 0.5421x + 2.5461, R2=0.999) and limit of detection (at S/N = 3) of 0.05 mg×L-1were received. In addition, this developed sensor provided high selectivity toward the reduction of gold ions without suffering from interferences. The proposed method has been successfully applied for the determination of Au ions in canal water sample (Saen Saep canal, Bangkok Thailand). The relative recovery and %RSD values were in the range of 92.77 – 107.51% and 2.17 – 4.15%, respectively. The results obtained can be compared with those of conventional methods (AAS or ICP-OES), however, the proposed sensor offers the advantages of fastness, simplicity, high sensitivity, and no requirement of complicated operational step. These approaches could be the alternative sensing for environmental filed monitoring. Keywords - Graphene Screen-Printed Electrode (SPGE), Gold Ions, Portable Sensor, Square Wave Voltammetry (SWV).

International Journal of Advances in Science Engineering and Technology, ISSN(p): 2321 –8991, ISSN(e): 2321 –9009 Vol-7, Iss-2, Spl. Issue-2 Jun.-2019

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Janus electrochemistry: Simultaneous electrochemical detection at multiple working conditions in a paper-based analytical device

Siriwan Nantaphol, Alyssa A.Kava , Robert B.Channon , TakeshiKondo , Weena Siangproh , Orawon Chailapakul , Charles S.Henry

https://doi.org/10.1016/j.aca.2019.01.026

Abstract

The simultaneous detection of multiple analytes from a single sample is a critical tool for the analysis of real world samples. However, this is challenging to accomplish in the field by current electroanalytical techniques, where tuning assay conditions towards a target analyte often results in poor selectivity and sensitivity for other species in the mixture. In this work, an electrochemical paper-based analytical device (ePAD) capable of performing simultaneous electrochemical experiments in different solution conditions on a single sample was developed for the first time. We refer to the system as a Janus-ePAD after the two-faced Greek god because of the ability of the device to perform electrochemistry on the same sample under differing solution conditions at the same time with a single potentiostat. In a Janus-ePAD, a sample wicks down two channels from a single inlet towards two discreet reagent zones that adjust solution conditions, such as pH, before flow termination in two electrochemical detection zones. These zones feature independent working electrodes and shared reference and counter electrodes, facilitating simultaneous detection of multiple species at each species’ optimal solution condition. The device utility and applicability are demonstrated through the simultaneous detection of two biologically relevant species (norepinephrine and serotonin) and a common enzymatic assay product (p-aminophenol) at two different solution pH conditions. Janus-ePADs show great promise as an inexpensive and broadly applicable platform which can reduce the complexity and/or number of steps required in multiplexed analysis, while also operating under the optimized conditions of each species present in a mixture.

Analytica Chimica Acta

Volume 1056, 16 May 2019, Pages 88-95

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Electrochemical detection of NOx gas based on disposable paper-based analytical device using a copper nanoparticles-modified screen-printed graphene electrode.

Kingkan Pungjunun, Sudkate Chaiyo, Narong Praphairaksit, Weena Siangproh, Astrid Ortner, Kurt Kalcher, Orawon Chailapakul , Eda Mehmeti

https://doi.org/10.1016/j.bios.2019.111606

Abstract

A disposable gas-sensing paper-based device (gPAD) was fabricated in origami design which integrates the gas adsorbent and the electrochemical detection zone in a single device. The gPAD for the determination of NOx gas uses a screen-printed graphene electrode modified with copper nanoparticles (CuNP/SPGE) to achieve high sensitivity and selectivity. The gPAD detects both, NO and NO₂ (as NOx) with same current responses. The measurement could be performed directly through differential pulse voltammetry (DPV) with a detection limit as low as 0.23 vppm and 0.03 vppm with exposure times of 25 min and 1 h, respectively. The reproducibility in terms of relative standard deviation was less than 5.1% (n = 7 devices) at 25, 75 and 125 vppm NO₂ and the life-time of this device was more than 30 days. The gPAD was applied to detect NOx in air and exhaust gases from cars. In comparison with spectrophotometry, there are no significant differences between both methods using a paired t-test of the results on a 95% confidence level. The designed gPAD can provide a new template model for other gas sensors with features of disposability and portability for fieldwork analysis at low cost.

Biosensors and Bioelectronics

Volume 143, 15 October 2019, 111606

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Development of glutamic acid and gold nanoparticles modified electrode for determination of arsenic

Sattakamon Kruekaew , Weena Siangproh

Abstract:

The electrochemical sensor using electrochemically co-depositing glutamic acid (GA) and gold nanoparticles (AuNPs) on a screen-printed graphene electrode (AuNPs-PGA/SPGE) was developed for the determination of arsenic using differential pulse anodic stripping voltammetry. Firstly, GA and AuNPs were deposited onto SPGE by cyclic voltammetry to obtain the layer of poly-glutamic acid (PGA) and AuNPs on the SPGE surface. In the presence of arsenic, the interaction between PGA and arsenic occurred and provided a well-defined oxidation peak of arsenic at 0.19 volts. Affecting parameters on the performances of AuNPsPGA/SPGE were investigated such as amplitude, step potential, deposition potential and deposition time to obtain the optimal sensitivity for detection. Under the optimal conditions, the developed sensor showed the linear range from 25 to 125 µM with detection limit 1 µM was achieved. This proposed sensor offers the possibility to use as an alternative sensor for determination of arsenic in real sample.

The 2019 Pure and Applied Chemistry International Conference (PACCON 2019)

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Colorimetric sensor for determination of phosphate ions using anti-aggregation of 2-mercaptoethanesulfonate-modified silver nanoplates and europium ions

Chanika Pinyorospathum , Poomrat Rattanarat , Sudkate Chaiyo , Weena Siangproh , Orawon Chailapakul

https://doi.org/10.1016/j.snb.2019.03.059

Abstract

A new, distinctive, and inexpensive colorimetric sensor for the detection of phosphate ions (Pi) performed on paper-based analytical devices (PADs) based on the anti-aggregation of 2-mercaptoethanesulfonate (MS)-modified silver nanoplates is proposed. An aggregation of MS-AgNPls due to the electrostatic interaction between the negatively charged sulfonate group on MS-AgNPls and europium ions (Eu³⁺) causes a color change. Conversely, the color of MS-AgNPls remains unchanged in the presence of premixed Eu³⁺ with Pi because of the higher binding affinity that Eu³⁺ has towards Pi, leaving the AgNPls dispersed. An apparent color change from brown to red with the increasing amount of Pi can be observed by the naked eye on PADs and can be used for quantification by the measurement of color intensity using ImageJ software and determined with the color comparator. The aggregation and anti-aggregation of MS-AgNPls were characterized by TEM, UV–vis spectroscopy, and FT-IR to confirm the mechanism. This method can detect Pi in the range of 1–30 mg L⁻¹, with a detection limit of 0.33 mg L⁻¹ (3SD/slope) and a limit of quantification equal to 1.01 mg L⁻¹ (10SD/slope). The optimum parameters, including pH, MS and Eu³⁺ concentrations, reaction time, and interference effects, were studied. This method can be applied with the detection of Pi in real samples collected from soils, and the results agree with those of the standard method (UV–vis spectroscopy).

Sensors and Actuators B: Chemical

Volume 290, 1 July 2019, Pages 226-232

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A Transparency Sheet-Based Colorimetric Device for Simple Determination of Calcium Ions Using Induced Aggregation of Modified Gold Nanoparticles

Paweenar Duenchay, Orawon Chailapakul, Weena Siangproh

^{https://pubmed.ncbi.nlm.nih.gov/31212937/}

Abstract

A simple and novel transparency sheet-based colorimetric detection device using gold nanoparticles (AuNPs) modified by 4-Amino-6-hydroxy-2-mercaptopyrimidine monohydrate (AHMP) was fabricated and developed for the determination of calcium ions (Ca²⁺). The detection was based on a colorimetric reaction as a result of the aggregation of modified AuNPs induced by Ca²⁺ due to the ability to form strong electrostatic interactions between positively charged Ca²⁺ and negatively charged modified AuNPs. Probe solution changes color from red to blue in the presence of Ca²⁺ and can be observed by the naked eyes. To verify the complete self-assembly of the AHMP onto the AuNP surface, the modified AuNPs were characterized using ultraviolet-visible spectroscopy and zeta potential measurements. Under optimal conditions, a quantitative linearity was 10 to 100 ppm (R² = 0.9877) with a detection limit of 3.05 ppm. The results obtained by the developed method were in good agreement with standard atomic absorption spectrometry (AAS) results and demonstrated that this method could reliably measure Ca²⁺. Overall, this novel alternative approach presents a low-cost, simple, sensitive, rapid, and promising device for the detection of Ca²⁺.

International journal of molecular sciences

Int J Mol Sci 2019 Jun 17;20(12):2954.

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3D Capillary-Driven Paper-Based Sequential Microfluidic Device for Electrochemical Sensing Applications

Abdulhadee Yakoh, Sudkate Chaiyo, Weena Siangproh, and Orawon Chailapakul

https://doi.org/10.1021/acssensors.8b01574

Abstract

This article describes the device design and fabrication of two different configurations (flow-through and stopped-flow) of a sequential fluid delivery platform on a microfluidic paper-based device. The developed device is capable of storing and transporting reagents sequentially to the detection channel without the need for external power. The device comprises two components: an origami folding paper (oPAD) and a movable reagent-stored pad (rPAD). This 3D capillary-driven device eliminates the undesirable procedure of multiple-step reagent manipulation in a complex assay. To demonstrate the scope of this approach, the device is used for electrochemical detection of biological species. Using a flow-through configuration, a self-calibration plot plus real sample analysis using a single buffer introduction are established for ascorbic acid detection. We further broaden the effectiveness of the device to a complex assay using a stopped-flow configuration. Unlike other electrochemical paper-based sensors in which the user is required to cut off the device inlet or rest for the whole channel saturation before measurement, herein a stopped-flow device is carefully designed to exclude the disturbance from the convective mass transport. As a proof of concept, multiple procedures for electrode modification and voltammetric determination of serotonin are illustrated. In addition, the research includes an impedimetric label-free immunosensor for α-fetoprotein using the modified stopped-flow device. The beneficial advantages of simplicity, low sample volume (1 μL), and ability to perform a complex assay qualify this innovative device for use with diverse applications.

American Chemical Society sensors

ACS Sens. 2019, 4, 5, 1211–1221

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An origami paper-based electrochemical immunoassay for the C-reactive protein using a screen-printed carbon electrode modified with graphene and gold nanoparticles

Suchanat Boonkaew, Sudkate Chaiyo, Sakda Jampasa, Sirirat Rengpipat, Weena Siangproh & Orawon Chailapakul

https://link.springer.com/article/10.1007%2Fs00604-019-3245-8

Abstract

An origami paper-based electrochemical immunoassay for C-reactive protein (CRP) detection is described. The assay integrates multiple steps of electrode modification into a single device. A graphene-modified screen-printed carbon electrode (G/SPCE) was employed to enhance sensitivity. Gold nanoparticles were first electrodeposited onto the G/SPCE, followed by a self-assembled monolayer of L-cysteine. The capture anti-CRP was then covalently immobilized on the modified electrode. CRP was quantified by measuring the changes in the charge-transfer resistance of the electrode by using hexacyanoferrate as the redox probe. Cyclic voltammetry and scanning electron microscopy were also applied to verify the successful modification of the electrode. Under optimal conditions, impedance increase in the 0.05-100 μg mL^-1 CRP concentration range, and the limit of detection is 15 ng mL^-1 (at S/N = 3). The immunoassay was successfully applied to the determination of CRP in a certified human serum sample. This method is simple, low-cost, portable and disposable. Graphical abstract An origami paper-based analytical device (oPAD) is described that integrates the multistep of electrode modification, immobilization and detection into a single device. The direct conjugation between the capture antibody and target molecule was allowed to use in this system. The C-reactive protein (CRP) concentration in serum samples was determined using electrochemical impedance spectroscopy.

Microchimica Acta volume 186, Article number: 153 (2019)

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Simultaneous determination of β-agonists by UHPLC coupled with electrochemical detection based on palladium nanoparticles modified BDD electrode

Atchara Lomae , Siriwan Nantaphol , Takeshi Kondo , Orawon Chailapakul , Weena Siangproh , Janjira Panchompoo

https://doi.org/10.1016/j.jelechem.2019.04.003

Abstract

Palladium nanoparticles (PdNPs) modified boron-doped diamond (BDD) electrode was simply fabricated via electrodeposition technique for the sensitive electro-analysis of four β-agonist compounds, including terbutaline (TER), salbutamol (SAL), ractopamine (RAC), and clenbuterol (CLB) after a prior separation by ultra-high performance liquid chromatography (UHPLC). The separation was carried out using a reverse phase C18 column with gradient elution of appropriate proportion between methanol and phosphate buffer solution (PBS) pH 7.0 at a flow rate of 1.0 min mL⁻¹. The following electrochemical detection (ECD) was accomplished by amperometric method with a detection potential of 1.0 V vs Ag/AgCl. The modified BDD electrode, unlike other carbon-based electrodes used for β-agonists detection, typically showed the superior anti-fouling ability and provided excellent long-term stability, resulting in no complicated cleaning steps needed during each determination. The measurement of β-agonists using the PdNPs modified BDD electrode integrated with UHPLC showed a linear relationship in the range of 0.2 to 200 μg mL⁻¹ with detection limits of 0.04, 0.02, and 0.03 μg mL⁻¹ for TER, SAL, and RAC, respectively. Meanwhile, CLB displayed linearly in the range of 0.5 to 200 μg mL⁻¹ with a detection limit of 0.19 μg mL⁻¹. Moreover, this method was successfully applied to detect β-agonists in diverse samples, including swine feed, swine meat, and human urine with satisfied recoveries in the range from 80.5% to 110.0%. Reliability of this developed method was also validated with ultra-high performance liquid chromatography coupled with ultra-violet detection (UHPLC-UV), and the results showed highly quantitative agreement between the two methods. Therefore, the PdNPs modified BDD could be productively utilized as effective electrodes, with good electrocatalytic activity of PdNPs towards β-agonists oxidation and favorable anti-fouling performance of BDD. The simultaneous detection of four β-agonists by UHPLC-ECD exhibited good stability, acceptable reusability, high sensitivity and rapid analysis.

Journal of Electroanalytical Chemistry

Volume 840, 1 May 2019, Pages 439-448

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Successive detection of benzoic acid and total parabens in foodstuffs using mercaptosuccinic acid capped cadmium telluride quantum dots

Kanlaya Prapainop, Wid Mekseriwattana, Weena Siangproh, Orawon Chailapakul, Kriangsak Songsrirote

https://doi.org/10.1016/j.foodcont.2018.10.009

Abstract

An approach for the determination of benzoic acid (BA) and total paraben content using mercaptosuccinic acid capped CdTe quantum dots (MSA-CdTe QDs) as an optical probe is presented. The levels of these preservatives in the samples of liquid foodstuff could be determined by both visible and fluorescent detection methods. For the analysis of paraben content, sample hydrolysis under alkaline conditions was required in order to obtain p-hydroxybenzoic acid (PHBA) prior to the addition of MSA-CdTe QDs. Both BA and PHBA could be quantified by their quenching of QD fluorescence, owing to the formation of hydrogen bonds between the carboxylic acid groups of the MSA-CdTe QDs and the analytes. Reverse-phase C18 solid phase extraction cartridge was exploited to reduce sample loss and organic solvent consumption in the sample preparation steps. The developed approach was successfully applied for the determination of the BA and total paraben content found in orange juice, soft drink, energy drink, beer, coconut milk, and fish sauce, giving a limit of detection of 0.3 mg/L for BA analysis, and 0.1 mg/L for PHBA (hydrolyzed form of parabens) analysis, with a linearity range from 1.0 mg/L to 500.0 mg/L. Since the concentrations of most preservatives added to products are controlled and regulated by raw, therefore, quality control is necessary to monitor the levels of these agents in products to better ensure the safety of customers.

Food Control

Volume 96, February 2019, Pages 508-516

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A new electrochemical paper platform for detection of 8-hydroxyquinoline in cosmetics using a cobalt phthalocyanine-modified screen-printed carbon electrode

Siriwan Nantaphol, Whitchuta Jesadabundit, Orawon Chailapakul, Weena Siangproh

https://doi.org/10.1016/j.jelechem.2018.11.055

Abstract

An electrochemical paper-based analytical device (ePAD) using an electrocatalytic cobalt(II) phthalocyanine-modified screen-printed carbon electrode (CoPc-SPCE) was first developed for the determination of 8-hydroxyquinoline (8-HQ). The electrochemical behavior of 8-HQ on the CoPc-modified electrode was investigated by cyclic voltammetry (CV) and square-wave voltammetry (SWV). The obtained results demonstrated that the CoPc-SPCE shows high electrocatalytic activity toward the oxidation of 8-HQ. The oxidation potential was shifted to a negative value compared to those obtained from the unmodified electrode. Under the optimized experimental conditions, a good linear relationship between the 8-HQ concentration and the peak current was obtained within the range of 10–250 μM with a detection limit of 0.89 μM. Furthermore, the method was successfully applied for the determination of 8-HQ in cosmetic samples. According to a paired t-test, the results were in good agreement with results from the standard HPLC-UV method. The benefits of this proposed device are that it is easily fabricated, low cost, disposable, highly sensitive and reproducible.

Journal of Electroanalytical Chemistry

Volume 832, 1 January 2019, Pages 480-485

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Disposable paper-based electrochemical sensor using thiol-terminated poly (2-methacryloyloxyethyl phosphorylcholine) for the label-free detection of C-reactive protein

Chanika Pinyorospathum, Sudkate Chaiyo, Pornpen Sae-ung, Voravee P. Hoven, Panittha Damsongsang, Weena Siangproh & Orawon Chailapakul

https://doi.org/10.1007/s00604-019-3559-6

Abstract

A paper-based electrochemical sensor is described that is based on the use of thiol-terminated poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC-SH) that was self-assembled on a gold nanoparticle-modified screen-printed electrode (SPE). The SPE sensor was used for label-free detection of C-reactive protein (CRP). Gold nanoparticles (AuNPs) were first electrodeposited on the SPCE, followed by the self-assembly of PMPC-SH on gold. The electrochemical response of the modified SPE to CRP was measured by differential pulse voltammetry (DPV). If the CRP on the paper device is contacted with Ca (II) ions, the current (measured by using hexacyanoferrate as the electrochemical probe) decreases. The signal drops in the 5 to 5000 ng·mL^-1 CRP concentration range, and the lower detection limit (at 3 SD/slope) is 1.6 ng·mL^-1. The use of a PMPC-modified surface also reduces the nonspecific adsorption of proteins. The sensor is not interfered by bilirubin, myoglobin and albumin. It was successfully applied to CRP detection in certified human serum. This sensor is applicable as an attractive protocol for an inexpensive, highly sensitive, and disposable material for electrochemical detection of CRP. Graphical abstract Schematic presentation of highly sensitive and disposable paper-based electrochemical sensor using thiol-terminated poly(2-methacryloyloxyethyl phosphorylcholine) in the presence of Ca²⁺ for the label-free C-reactive protein detection. The current was measured by differential pulse voltammetry

Microchimica Acta volume 186, Article number: 472 (2019)

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The Synthesis of Carboxymethyl Cellulose‐Based Hydrogel from Sugarcane Bagasse Using Microwave‐Assisted Irradiation for Selective Adsorption of Copper (II) Ions

Chanathip Baiya, Loetsak Nannuan, Yosthanase Tassanapukdee, Orawon Chailapakul, Kriangsak Songsrirote

https://doi.org/10.1002/ep.12950

Abstract

This study presents the sustainable applications of recycling agricultural waste. The cellulose from sugarcane bagasse (SCB) was extracted to prepare the carboxymethyl cellulose (CMC) used as the starting material for synthesizing CMC‐based hydrogel for the selective adsorption of Cu(II) ions in contaminated solution. Hydrogel fabrication was performed under microwave‐assisted irradiation, which helps to improve reaction efficiency and significantly reduces reaction time compared with using a conventional hotplate. The hydrogel of 25% CMC blended with poly(vinyl alcohol) (PVA) and using glutaraldehyde (GA) as a cross‐linking agent showed the property of selective adsorption for Cu(II) ions from the solution with an adsorption capacity of 2.3 mg/g (92.4% removal) under ambient temperature with pH 5.0. The adsorbed Cu(II) ions were effectively desorbed from the hydrogel by either 0.1 M HCl or 0.1 M EDTA. Thus, the prepared hydrogel could potentially be applied in the removal of Cu(II) ions from contaminated water, with promising potential for reuse after the regeneration process. Sugarcane bagasse was used as a starting material for the synthesis of hydrogel, which has selective adsorption property to Cu(II) ions. In addition, microwave‐assisted irradiation, technology that is able to save time and energy, was exploited for the polymer synthesis. Therefore, this study presents the sustainable applications of recycling agricultural waste for the purpose of water treatment.

Environmental Progress & Sustainable Energy

American Institute of Chemical Engineers Environ Prog, 38: S157–S165, 2019

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Simultaneous determination of ethanol and total sulfite in white wine using on-line cone reservoirs membraneless gas-liquid separation flow system

Pitchnaree Kraikaew, Thanakorn Pluangklang, Nuanlaor Ratanawimarnwong, Kanchana Uraisin, Prapin Wilairat, Thitirat Mantim , Duangjai Nacapricha

https://doi.org/10.1016/j.microc.2019.104007

Abstract

This work presents the use of a single membraneless vaporization unit (MBL-VP unit) in a flow system for the simultaneous determination of ethanol and total sulfite in white wine. The flow system comprises a MBL-VP unit with three cone-shaped reservoirs and two in-house detectors, a paired emitter-detector diodes (PEDD) and a capacitively coupled contactless conductivity detector (C4D). The sample of white wine is first acidified in the flow system. Then 200-μL of the acidified sample is delivered to the donor reservoir of the MBL-VP unit in which the two acceptor reservoirs contain 200-μL of the gas acceptor reagents, viz. acidic permanganate solution and deionized water. Vaporization of ethanol and SO_2(g) (converted from sulfite) from the donor into the acceptor reservoirs is carried out for 15 s. The two acceptor solutions are then simultaneously transferred for separate detection at the PEDD and C4D detectors. Decolorization of the permanganate solution by the reduction reaction with absorbed ethanol gives an increase in the PEDD signal, whereas dissolution of the SO_2(g) in the water acceptor leads to an increase in conductivity and detected by the C4D. Linear calibrations were obtained in the range of 5.0–15.0% (v/v) for ethanol (PEDD signal = ((4.0 ± 0.03) × 10⁻²) · (%(v/v) ethanol) – ((1.85 ± 0.35) × 10⁻²): r² = 0.999) and 10–200 mg L⁻¹ for sulfite (C4D signal = ((0.64 ± 0.015) × 10⁻²) · (mg L⁻¹ sulfite) – ((3.25 ± 1.64) × 10⁻²): r² = 0.999). The analysis is rapid with total analysis time of 2.5 min. Percentage of recoveries were 81–104% and 88–110% for ethanol and sulfite, respectively. The method was compared with gas chromatographic analysis and iodometric titration for ethanol and sulfite, respectively, with no statistically significant difference for the analysis of seven white wine samples.

Microchemical Journal

Volume 149, 2019, 104007

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T-shirt ink for one-step screen-printing of hydrophobic barriers for 2D-and 3D-microfluidic paper-based analytical devices

Jirayu Sitanurak, Nutnaree Fukana, Thinnapong Wongpakdee, Yanisa Thepchuay, Nuanlaor Ratanawimarnwong, Taweechai Amornsakchai, Duangjai Nacapricha

https://doi.org/10.1016/j.talanta.2019.120113

Abstract

This work presents the use of polyvinyl chloride (PVC) fabric ink, commonly employed for screening t-shirts, as new and versatile material for printing hydrophobic barrier on paper substrate for microfluidic paper-based analytical devices (μPADs). Low-cost, screen-printing apparatus (e.g., screen mesh, squeegee, and printing table) and materials (e.g. PVC ink and solvent) were employed to print the PVC ink solution onto Whatman filter paper No. 4. This provides a one-step strategy to print flow barriers without the need of further processing except evaporation for 3–5 min in a fume hood to remove the solvent. The production of the single layer μPADs is reasonably high with up to 77 devices per screening with 100% success rate. This method produces very narrow fluidic channel 486 ± 14 μm in width and hydrophobic barrier of 642 ± 25 μm thickness. Reproducibility of the production of fluidic channels and zones is satisfactory with RSDs of 2.9% (for 486-μm channel, n = 10), 3.7% (for 2-mm channel, n = 50) and 1.5% (for 6-mm diameter circular zone, n = 80). A design of a 2D-μPAD produced by this method was employed for the colorimetric dual-measurements of thiocyanate and nitrite in saliva. A 3D-μPADs with multiple layers of ink-screened paper was designed and constructed to demonstrate the method's versatility. These 3D-μPADs were designed for gas-liquid separation with in-situ colorimetric detection of ethanol vapor on the μPADs. The 3D-μPADs were applied for direct quantification of ethanol in beverages and highly colored pharmaceutical products. The printed barrier was resistant up to 8% (v/v) ethanol without liquid creeping out of the barrier.

Talanta

Volume 205, 1 December 2019, 120113

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Chromogenic Detection of Fe²⁺ Using Schiff base–naphthalene-2-ol-modified Silver Nanoparticles

Wachirawit Samerjai, Lalita Dankhanob, Parisa Chotimai, Piyada Jittangprasert & Pan Tongraung

https://doi.org/10.1007/s40995-017-0425-4

Abstract

Schiff base–naphthalene-2-ol (L) was synthesized by a coupling reaction between 2-hydroxy-1-naphthaldehyde and xylylenediamine in ethanol. The product was characterized by ¹H NMR, ¹³C NMR, and mass spectroscopy. L was utilized as reducing and surface functionalizing agents in the synthesis of silver nanoparticles (AgNPs) which were then characterized by high-resolution transmission electron microscopy (HR-TEM) and UV–Visible spectroscopy. The colorimetric sensitivity and metal ion selectivity of L-modified AgNPs with various cations were examined using UV–Vis spectrophotometry. The presence of Fe²⁺ induced the aggregation of AgNPs through a cooperative metal–ligand interaction, resulting in a color change from bright yellow to dark yellow with a new band at 372 nm. The interference studies confirmed the high selectivity of Fe²⁺ sensing in presence of cations as well as anions by L-modified AgNPs. A linear relationship between the change of absorption at 372 nm and concentration Fe²⁺ ranging from 1.30 × 10⁻⁵ to 1.13 × 10⁻³ M was observed with a correlation coefficient of 0.9949. The detection limit of the developed method was 1.06 × 10⁻⁶ M. The proposed method has been successfully applied for determination of Fe²⁺ content in multivitamin tablet samples. The results were in good agreement with the label claim.

Iranian Journal of Science and Technology, Transactions A: Science volume 43, pages451–456(2019)

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Simple Method for Monitoring Melamine in Food by Ion Pair Chromatography

Jaruwan Chutrtong , Waradoon Chutrtong

https://doi.org/10.1016/j.promfg.2019.02.314

Abstract

A simple, rapid, precise and accurate, ion-pair reversed-phase high-performance liquid chromatography (HPLC) method is developed to determine melamine in a wide range of food matrices, including several milk-based products. The sample is prepared by protein precipitation with 6M hydrochloric and methanol (1:9). As melamine is present in supernatant in the cationic form, the chromatographic separation in reversed-phase LC requires the use of anionic ion pair reagent, such as 1-Hexanesulfonic acid sodium. This allows a satisfactory chromatographic retention and peak shape in all type of food samples investigated. The separation is performed on a Vetical VertiSepᵀᴹ pH endure C18 column (4.6 id x 250 mm, 5 µm particle size) at room temperature. The mobile phase (0.01 M 1-Hexanesulfonic acid sodium in 50 mM NaH2PO4) is pump at a flow rate of 1.0 ml/min with detection at 215 nm. Melamine elutes at 5.4 min. A linear response (r = 0.999) is observed for sample ranging from 5-20 µg/mL. The method provides recoveries of 97.8-100.7% (mean=99.4) with the RSDs (n=5) of 3.34-3.82% in the concentration 5 - 20 µg/mL. The limit of detection (LOD) and limit of quantitation values is 0.16 and 0.53 µg/mL(s/n=5) respectively.

Procedia Manufacturing

Volume 32, 2019, Pages 1000-1007

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Paper for Chromatographic Technique from Coconut Pulp Cellulose

Jaruwan Chutrtong, Waradoon Chutrtong

https://doi.org/10.1016/j.promfg.2019.02.310

Abstract

Cellulose is an important component of green plants cell wall and other organisms. Cellulose from plants is mainly used to produce paper. Because of the widespread use of paper throughout the world, it causes the use of wood in large quantities that involves cutting down trees which can lead to negative impacts on ecosystems. Many research studied to find other raw materials that could replace wood and some focused on coconut coir because it contains high amount of cellulose which is enough to use in manufacturing. Coconut pulp is also the residue from coconut. It has 59 % cellulose. This makes it possible to use coconut pulp in paper industry. Paper for chromatography uses fiber from wood as an important component too. So, the purpose of this study was to identify whether cellulose from coconut pulp can replace the original material of chromatography paper. The procedure started from extracted impurity from coconut pulp and used the remaining for making chromatographic paper. Washed coconut pulp and removed residue coconut milk. Boiled clean coconut pulp for 10 minutes and dried in oven at 60 degree Celsius. Remove the residue fat with hexane. Drained hexane and dried in oven at 60 degree Celsius. Extracted cellulose by the soda process. Cellulose yield was 50.85%. Used extracted cellulose as raw material for making chromatographic paper. Tried different amount of cellulose per area of sieve to find the suitable for making paper. The appropriate paper area per extracted cellulose weight was about 81.25 cm²/g. Then trailed the paper used as stationary phase for color separation. The appropriate condition of mobile phase in chromatographic technique (ethyl-methyl ketone, acetone and water) to separate standard colors was also studied. The appropriate ratio of mobile phase which could separate standard solutions was 7:3:3, respectively. The results show that this paper was effective in serving as a stationary phase. This paper has the potential to be developed to use as a substitute material in Bio manufacturing.

Procedia Manufacturing

Volume 32, 2019, Pages 969-974

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Simultaneous and direct determination of urea and creatinine in human urine using a cost-effective flow injection system equipped with in-house contactless conductivity detector and LED colorimeter

Sumonmarn Chaneam, Kamparnart Kaewyai, Thitirat Mantim, Rasamee Chaisuksant, Prapin Wilairat, Duangjai Nacapricha

https://doi.org/10.1016/j.aca.2019.05.003

Abstract

This work presents a cost-effective and simple flow injection analysis (FIA) system for simultaneous and direct determination of urea and creatinine in human urine. The FIA system comprises two in-house detectors, a contactless conductivity detector and a light emitting diode (LED) detector. The contactless detector was built as a flow-through detection cell with axial electrodes, commonly known as capacitively coupled contactless conductivity detector (C4D) and the diode detector was fabricated based on the concept of paired emitter detector diodes (PEDD). With appropriate dilution of urine, the sample is directly injected into a stream of glycine-NaOH buffer pH 8.8 (the gas donor stream) and is carried by the carrier through a urease minicolumn for on-line enzymatic hydrolysis. The generated NH₃ diffuses from the carrier stream through a porous polytetrafluoroethylene (PTFE) membrane into a stream of deionized water (the acceptor stream) leading to an increase in the signal at the C4D due to the NH₃ dissolution in the water. In this system, creatinine is determined based on the Jaffé reaction by merging a stream of alkaline picrate with the gas donor stream. The change in color is detected using the PEDD equipped with two green LEDs. Under the optimum condition, the linear range of urea and creatinine were 30–240 mg L⁻¹ and 10–500 mg L⁻¹, with limits of detection of 9.0 mg L⁻¹ and 0.9 mg L⁻¹, respectively. The proposed system provides satisfactorily good precision (RSD < 3%), with sample throughput of 31 sample h⁻¹ for the two analytes. The FIA system tolerates potential interference commonly found in human urine. The system was successfully applied and validated with selected reference methods.

Analytica Chimica Acta 1073 (27) 2019, Pages 54-61

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Scandium (III)‐Triflate‐Catalyzed Pinacol‐Pinacolone Rearrangement and Cyclization of 1, 2‐Diaryl‐1, 2‐Ethanediol: A Versatile Synthesis of 1‐Aryl‐2, 3‐Dihydro‐1H‐3‐Benzazepines

Winai Ieawsuwan, Ratchanok Pingaew, Sukanya Kunkaewom, Poonsakdi Ploypradith, Somsak Ruchirawat

https://doi.org/10.1002/ajoc.201900318

Abstract

An efficient scandium(III)‐triflate‐catalyzed pinacol‐pinacolone rearrangement and cyclization of 1,2‐diaryl‐1,2‐ethanediol has been developed, providing a variety of 2,3‐dihydro‐1H‐3‐benzazepine derivatives in good yields. The obtained products could be transformed to other valuable building blocks for the synthesis of some biologically active compounds or natural products. Moreover, this protocol was also successfully employed for the synthesis of the corresponding 1‐phenyl‐3,4,5,6‐tetrahydrobenzo[d]azocine and 3‐phenylisoquinoline products.

Asian Journal of Organic Chemistry

Asian J. Org. Chem. 8(8) 2019, 1441-1447.

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Synthesis, molecular docking, and QSAR study of bis-sulfonamide derivatives as potential aromatase inhibitors

Ronnakorn Leechaisit , Ratchanok Pingaew , Veda Prachayasittikul , Apilak Worachartcheewan , Supaluk Prachayasittikul , Somsak Ruchirawat , Virapong Prachayasittikul

https://doi.org/10.1016/j.bmc.2019.08.001

Abstract

A library of bis-sulfonamides (9–26) were synthesized and tested for their aromatase inhibitory activities. Interestingly, all bis-sulfonamide derivatives inhibited the aromatase with IC₅₀ range of 0.05–11.6 μM except for compound 23. The analogs 15 and 16 bearing hydrophobic chloro and bromo groups exhibited the potent aromatase inhibitory activity in sub-micromolar IC₅₀ values (i.e., 50 and 60 nM, respectively) with high safety index. Molecular docking revealed that the chloro and bromo benzenesulfonamides (15 and 16) may play role in the hydrophobic interaction with Leu477 of the aromatase to mimic steroidal backbone of the natural substrate, androstenedione. QSAR study also revealed that the most potent activity of compounds was governed by van der Waals volume (GATS6v) and mass (Mor03m) descriptors. Finally, the two compounds (15 and 16) were highlighted as promising compounds to be further developed as novel aromatase inhibitors.

Bioorganic & Medicinal Chemistry

Volume 27, Issue 19, 1 October 2019, 115040

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Development of antituberculosis melt-blown polypropylene filters coated with mangosteen extracts for medical face mask applications

Pongpol Ekabutr, Piyachat Chuysinuan, Sunit Suksamrarn, Wasana Sukhumsirichart, Poonpilas Hongmanee & Pitt Supaphol

https://doi.org/10.1007/s00289-018-2468-x

Abstract

The aim of this article was to develop a three-layer mask, which was made of a polypropylene filter containing mangosteen extract by spray-coating technique in order to enhance antibacterial and antituberculosis activities. The bacterial filtration efficiency was performed by spraying the biological aerosol through the filters. Breathability of face masks was also measured as a pressure drop parameters. The physical properties of filters were evaluated in terms of surface morphology and water contact angle. The coated filters were then challenged with multidrug-resistant tuberculosis, Staphylococcus aureus and Escherichia coli as the representative bacteria. The results showed that the increase in the mangosteen extract concentration for coating caused fiber diameter, hydrophilicity, % BFE (> 95%) and pressure drop of filters to be also increased. Investigation into release characteristic of mangosteen extract-coated polypropylene filters exhibited initial burst release after 60 min of immersion in a phosphate buffer solution. The coated filter exhibited good antibacterial performances against three types of pathogens. An in vitro cytotoxic test showed that 2% and 5% w/v mangosteen extract-coated polypropylene filters were not toxic by an indirect cytotoxicity test using L929 mouse fibroblast cells. This study demonstrated that the filters coated with mangosteen extract significantly play an important role in achieving antibacterial face mask.

Polymer Bulletin volume 76, pages1985–2004(2019)

The inhibitory effect of 13-butoxyberberine bromide on migration in breast cancer MDA-MB-231 cells

Laomethakorn, P., Jaitrong, M., Samosorn, S., Watanapokasin, R.

http://www.jmatonline.com

Abstract

13-butoxyberberine bromide is a berberine derivative that has not been reported on the antimigration mechanism of breast cancer . Cancer metastasis is the major cause of cancer mortality, about 90% of cancer patients death are caused by cancer metastasis not the primary tumor. Therefore, the compound that could inhibit cancer migration is necessary for development as anti- cancer drug. Objective: To investigate the effects of 13-butoxyberberine bromide on the anti- migration of breast cancer MDA-MB-231 cells . Materials and Methods: Cell viability was determined by MTT assay. Wound-healing assay and transwell chamber assay were used to determine cell migration . Protein expression levels were examined by western blot analysis. Results: 13- Butoxyberberine bromide at sub-toxic concentration (10 μg/mL) inhibited cell migration in MDA-MB-231 treated cells via down-regulation of MMP-2 and MMP-9 expression which play important role in cancer cell metastasis. Conclusion: 13-Butoxyberberine bromide at 10 μg/mL showed anti- migration effect against MDAMB-231 cells through inhibition of mediator proteins. These findings could be useful in the development of 13- butoxyberberine bromide to be a novel anti- migration agent for clinical use in the future.

Journal of the medical association of Thailand

J Med Assoc Thai 2019;102(Suppl.6): 12-16

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A new pyrrole alkaloid from the roots of Cissampelos pareira

Thitima Rukachaisirikul, Sittisak Kumjun, Parichat Suebsakwong, Nuttapon Apiratikul, Apichart Suksamrarn

https://doi.org/10.1080/14786419.2019.1614576

Abstract

Phytochemical investigation of the roots of Cissampelos pareira Linn. led to the isolation of one new pyrrole alkaloid, cissampeline (1), together with ten known alkaloids, (−)-curine (2), (−)-cyclanoline (3), (+)-tetrandrine (4), (+)-obaberine (5), (+)-obamegine (6), (−)-oblongine (7), (+)-homoaromoline (8), (−)-nor-N׳-chondrocurine (9), trans-N-feruloyltyramine (10) and (+)-coclaurine (11). Their structures were elucidated by extensive NMR and MS spectroscopic analyses. Interestingly, compound 1 represents the first example of pyrrole alkaloid found in the genus Cissampelos. Moreover, compounds 5-11 were isolated for the first time from this genus. Among them, compound 6 showed the highest anti-acetylcholinesterase activity with an IC₅₀ value of 3.26 µM, whereas compound 8 displayed the most potent cytotoxicity against human colon cancer (HT29) cells with an IC₅₀ value of 7.89 µM.

Natural product research 2019, 3-8

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Application of deuterated THENA for assigning the absolute configuration of chiral secondary alcohols

Jakapun Soponpong, Kulvadee Dolsophon, Chawanee Thongpanchang, Anthony Linden, Tienthong Thongpanchang

https://doi.org/10.1016/j.tetlet.2019.01.013

Abstract

The structure of a constrained bicyclic chiral derivatizing agent (CDA), 1,2,3,4-tetrahydro-1,4-epoxynaphthalene-1-carboxylic acid, THENA 1, was modified by replacing both exo-methylene protons with deuterium atoms. The modified CDA, THENA-d₂ 2, could be used to assign the absolute configuration of chiral secondary alcohols with good reliability. Compared with THENA, the multiplicity of the methylene proton signals in the ¹H NMR spectra of THENA-d₂ derivatives is less complicated and the new CDA thus offers simpler NMR spectra for data interpretation.

Tetrahedron letters

Tetrahedron Letters

Volume 60, Issue 6, 7 February 2019, Pages 497-500

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The Study of Graduate Students’ Effectiveness and Attitude towards ‘English Mate’

Abstract

The objectives of this study were as follows: 1) the management and problems found when using English Mate as a supplementary material to improve the English language skills of postgraduate university students; 2) the development of English skills and attitudes towards students using English Mate: and 3) to develop documentary film media for English self-learning among the students. The samples in this study included thirty-three graduate students who registered for the language proficiency test in the 2017 academic year. The research instruments consisted of a questionnaire designed to measure the satisfaction of

the students with English Mate and followed up with in-depth interviews. The data were analyzed by percentage. The results of the research found that: 1) the English Mate program requires a computer with a DVD players, but satisfaction with English Mate was at a high level and that this learning medium should be used as a supplementary material in learning English, 2) the most developed language skills were expressions and vocabulary, listening, reading and culture, while speaking and writing skills were developed at a medium level; and 3) a TED Talks lecture was developed as supplementary material for graduate classes.

Journal of Research and Curriculum Development

Vol. 9 No. 2 July – December 2019, 114-124.

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Effects of Zn(II) on hydrogen peroxide-induced corrosion of stainless steel

S Makjan, P Boonsri, J Channuie and K Kanjana

https://doi.org/10.1088/1742-6596/1285/1/012045

Abstract

Zinc injection has been widely applied in nuclear industry for corrosion mitigation of nuclear materials. The corrosion resistance mechanism of zinc in the presence of the radiolytic oxidizing species is complex and has not been completely understood. Without such information it is not possible to improve the protocol. In the present study, zinc effects on corrosion of 304 SS exposed to H₂O₂ at 200-1000 ppb for 7 and 70 hrs were investigated at room temperature using a custom-made flow setup. XRF and SEM-EDX were used to examine the chemical composition and surface morphology of the specimens. The results indicated that pitting was the dominant form of corrosion found under the experimental conditions. Zinc ions tend to help mitigate corrosion of the material via playing a significant role in oxide formation on the steel surface.

Journal of Physics: Conf. Series

J. Phys.: Conf. Ser. Volume 1285, 2019, 1-8.

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Theoretical Study of the Electronic Structure and Properties of Alternating Donor-Acceptor of Carbazole- Based Copolymer for Advanced Organic Light-Emitting Diodes (OLED)

Suppamat Makjan, Malinee Promkatkaew, Supa Hannongbua, Pornthip Boonsri

https://doi.org/10.4028/www.scientific.net/KEM.824.236

Abstract

Generally, it is difficult to generate a high-performance pure blue emission organic light-emitting diode (OLED). That is because the intrinsically wide band-gap makes it hard to inject charges into the emitting layer in such devices. To solve the problem, carbazole derivatives have been widely used because they have more thermal stability, a good hole transporting property, more electron rich (p-type) material, and higher photoconductivity. In the present work, novel copolymers containing donor-acceptor-acceptor-donor (D-A-A-D) blue compounds used for OLEDs were investigated. The theory of the geometrical and electronic properties of N-ethylcarbazole (ECz) as donor molecule (D) coupled to a series of 6 acceptor molecules (A) for advanced OLEDs were investigated. The acceptors were thiazole (TZ), thiadiazole (TD), thienopyrazine (TPZ), thienothiadiazole (TTD), benzothiadiazole (BTD), and thiadiazolothienopyrazine (TDTP). The ground state structure of the copolymers were studied using Density Functional Theory (DFT) at B3LYP/6-31G(d) level. Molecular orbital analysis study indicated 3 investigated copolymers (ECz-diTZ-ECz, ECz-diTD-ECz, ECz-diBTD-ECz) have efficient bipolar charge transport properties for both electron and hole injection to the TiO₂ conduction band (4.8 eV). In addition, the excited states electronic properties were calculated using Time-Dependent Density Functional Theory (TD-DFT) at the same level. Among these investigated copolymer ECz-diTZ-ECz and ECz-diTD-ECz showed the maximum absorption wavelengths (λ_abs) with blue emitting at 429 and 431 nm, respectively. The results suggested that selected D-A-A-D copolymers can improve the electron- and hole- transporting abilities of the devices. Therefore, the designed copolymers would be a promising material for future development of light-emitting diodes, electrochromic windows, photovoltaic cells, and photorefractive materials.

Key Engineering Materials 824, 2019, 236-244.

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Structural and spectroscopic properties of metal complexes with Ruhemann’s purple compounds calculated using density functional

Malinee Promkatkaew, Pornthip Boonsri, Supa Hannongbua

https://doi.org/10.4028/www.scientific.net/KEM.824.204

Abstract

Structural and spectroscopic properties of Ruhemann’s purple (RP) and its transition metal coordination complexes were calculated using theoretical chemistry techniques. The obtained information described RP and its coordination complexes with the transition metal ions [Cr(II), Mn(II), Fe(II), Co(II), Ni(II), Cu(II), Zn(II), Cd(II) and Hg(II)]. The procedures involved calculations of what are called density functional theory (DFT) and time-dependent DFT (TD-DFT). These methods optimized what is called, in the codes of theoretical chemistry, the hybrid density B3LYP function employing the 6‐311++G(d,p) and LANL2DZ basis sets. The RP geometries, bond lengths, angles, quantum chemical parameters, and excitation spectra indicate that the RP is well able to coordinate with a transition element ion. Then the correlation of these theoretical results with experimental observations provides a detailed description of the structural and spectroscopic properties of RP compounds. The inclusion of solvent effects causes a blue shift in all theoretical excitation spectra. In summary, this work leads to an understanding of the characteristics of transition metal complexes with Ruhemann’s purple. These materials can be applied in forensic chemistry as reagents in developing latent fingerprints.

Key Engineering Materials 824:204-211

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Molecular Docking Studies and Synthesis of Amino-oxy-diarylquinoline Derivatives as Potent Non-nucleoside HIV-1 Reverse Transcriptase Inhibitors

Arthit Makarasen, Mayuso Kuno, Suwicha Patnin, Nanthawan Reukngam, Panita Khlaychan, Sirinya Deeyohe, Pakamas Intachote, Busakorn Saimanee, Suchada Sengsai, Pornthip Boonsri, Apinya Chaivisuthangkura, Wandee Sirithana, Supanna Techasakul

https://doi.org/10.1055/a-0968-1150

Abstract

In this study, amino-oxy-diarylquinolines were designed using structure-guided molecular hybridization strategy and fusing of the pharmacophore templates of nevirapine (NVP), efavirenz (EFV), etravirine (ETV, TMC125) and rilpivirine (RPV, TMC278). The anti-HIV-1 reverse transcriptase (RT) activity was evaluated using standard ELISA method, and the cytotoxic activity was performed using MTT and XTT assays. The primary bioassay results indicated that 2-amino-4-oxy-diarylquinolines possess moderate inhibitory properties against HIV-1 RT. Molecular docking results showed that 2-amino-4-oxy-diarylquinolines 8(A-D): interacted with the Lys101 and His235 residue though hydrogen bonding and interacted with Tyr318 residue though π-π stacking in HIV-1 RT. Furthermore, 8A: and 8D: were the most potent anti-HIV agents among the designed and synthesized compounds, and their inhibition rates were 34.0% and 39.7% at 1 µM concentration. Interestingly, 8A: was highly cytotoxicity against MOLT-3 (acute lymphoblastic leukemia), with an IC₅₀ of 4.63±0.62 µg/mL, which was similar with that in EFV and TMC278 (IC₅₀ 7.76±0.37 and 1.57±0.20 µg/ml, respectively). Therefore, these analogs of the synthesized compounds can serve as excellent bases for the development of new anti-HIV-1 agents in the near future.

Drug Research

Drug Res 69(12), 2019, 671-682.

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Novel triazole-tetrahydroisoquinoline hybrids as human aromatase inhibitors

Chanamon Chamduang, Ratchanok Pingaew, Veda Prachayasittikul, Supaluk Prachayasittikul, Somsak Ruchirawat, Virapong Prachayasittikul

https://doi.org/10.1016/j.bioorg.2019.103327

Abstract

Novel thirteen triazole-tetrahydroisoquinoline derivatives (2a-m) were synthesized and evaluated for their aromatase inhibitory activities. Seven triazoles showed significant aromatase inhibitory activity (IC₅₀ = 0.07–1.9 μM). Interestingly, the analog bearing naphthalenyloxymethyl substituent at position 4 of the triazole ring (2i) displayed the most potent aromatase inhibitory activity (IC₅₀ = 70 nM) without significant cytotoxicity to a normal cell. Molecular docking also suggested that the direct H-bonding interaction with residue Thr310 may be responsible for a striking inhibitory effect of the most potent compound 2i.

Bioorganic Chemistry

Volume 93, December 2019, 103327

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Repurposing of nitroxoline drug for the prevention of neurodegeneration

Truong Van Hau, Waralee Ruankham, Wilasinee Suwanjang, Napat Songtawee, Prapimpun Wongchitrat, Ratchanok Pingaew, Virapong Prachayasittikul, Supaluk Prachayasittikul and Kamonrat Phopin

https://doi.org/10.1021/acs.chemrestox.9b00183

Abstract

Oxidative stress has been documented as one of the significant causes of neurodegenerative diseases. Therefore, antioxidant therapy for the prevention of neurodegenerative diseases seems to be an interesting strategy in drug discovery. The quinoline-based compound, namely 5-nitro-8-quinolinol (NQ), has shown excellent antimicrobial, anticancer, and anti-inflammatory activities. However, its neuroprotective effects and precise molecular mechanisms in human neuronal cells have not been elucidated. In this work, the effects of NQ on cell viability and morphology were evaluated by the MTT assay and microscopic observation. Moreover, the underlying mechanisms of this compound, inducing the survival rate of neuronal cells under oxidative stress, were investigated by reactive oxygen species (ROS) assay, flow cytometry, Western blotting, and immunofluorescence techniques. In addition, the molecular interaction of sirtuin1 (SIRT1) with NQ was constructed using the AutoDock 4.2 program. Interestingly, NQ protected SH-SY5Y cells against H2O2-induced neurotoxicity through scavenging ROS, upregulating the levels of SIRT1 and FOXO3a, increasing the levels of antioxidant enzymes (catalase and superoxide dismutase), promoting antiapoptotic BCL-2 protein expression, and reducing apoptosis. Besides, molecular docking also revealed that NQ interacted satisfactorily with the active site of SIRT1 similar to the resveratrol, which is the SIRT1 activator and strong antioxidant. These findings suggest that NQ prevents oxidative-stress-induced neurodegeneration because of its antioxidant capacity as well as antiapoptotic property through SIRT1-FOXO3a signaling pathway. Thus, NQ might be a drug that could be repurposed for prevention of neurodegeneration.

Chemical Research in Toxicology

Chem. Res. Toxicol. 2019, 32, 11, 2182–2191

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A novel bead synthesis of the Chiron-sodium dodecyl sulfate hydrogel and its kinetics-thermodynamics study of superb adsorption of alizarin red S from aqueous solution

Nunticha Limchoowong, Phitchan Sricharoen & Saksit Chanthai

https://doi.org/10.1007/s10965-019-1944-9

Abstract

We present a novel strategy for one step synthesis of iron (III) hydroxide doped chitosan (“Chiron”) without using acidic solvent via sodium dodecyl sulfate (SDS) as surfactant gelation, namely, Chiron-SDS hydrogel bead. The Chiron-SDS is developed as a potentially attractive adsorbent for an investigation of the noxious anionic dye from aqueous solution. The bead formation was obtained from 20 μL of the Chiron solution, and their uniform bead diameter was observed about 179 ± 0.13 μm, of which containing 86.7% moisture or 6 × 10⁻⁵ g DW per bead. Alizarin red S (AR) was chosen as pollutant model and was monitored spectrophometrically at 425 nm. The bead size after AR adsorption was slightly larger (185 ± 0.10 μm) than that of its original one. Fourier transform infrared analysis indicated that the Fe (OH)₃ were chelated with chitosan structure in the Chiron-SDS beads. The equilibrium data fit to Langmuir as the best representative model (R² 0.99), and their kinetics data are well fitted with the pseudo-second order. It was found that the maximum adsorption capacity (q_m) from Langmuir model for AR by Chiron-SDS is 294 mg g⁻¹, which was much higher than those previously reported data. The calculated thermodynamic parameters show that the dye adsorption is spontaneous and endothermic process. The advantage characteristics of ease, low cost, eco-friendly, and superb high adsorption efficiency demonstrate that this output gives a great deal to step forward for a huge scale elimination of toxic dye contaminants from aqueous solution, leading usefulness further for an environmental remediation.

Journal of Polymer Research volume 26, Article number: 265 (2019)

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Enhanced photocatalytic degradation of methylene blue using Fe2O3/graphene/CuO nanocomposites under visible light

Prawit Nuengmatcha, Paweena Porrawatkul, Saksit Chanthai, Phitchan Sricharoen, Nunticha Limchoowong

https://doi.org/10.1016/j.jece.2019.103438

Abstract

A visible light-responsive photocatalyst of Fe₂O₃/graphene/CuO (FGC) nanocomposite was successfully synthesized via a simple solvothermal method. The characteristics of the as-prepared graphene/mixed metal oxides were examined by XRD, SEM, EDS, TEM, and HR-TEM, and its magnetic property was evaluated by VSM. The analysis clearly revealed that the FGC hybrid catalyst, having a bandgap of 1.82 eV, has the ability to absorb visible light as observed from the UV–vis diffuse reflectance spectrum. The photocatalytic performance of FGC, subjected to visible light, in degrading methylene blue was evaluated. Its photocatalytic degradation property was found to be higher in the presence of visible light as compared to the other catalysts. In addition, the postulated mechanism of the photocatalytic property of this graphene/mixed metal oxides hybrid composite has been discussed. The present work demonstrates that this graphene/metal oxide hybrid nanocomposite can also be applied as a highly potent photocatalyst degrader for other dye pollutants.

Journal of Environmental Chemical Engineering

Volume 7, Issue 6, December 2019, 103438

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Creating Flavin Reductase Variants with Thermostable and Solvent-Tolerant Properties by Rational-Design Engineering

Somchart Maenpuen, Vinutsada Pongsupasa, Wiranee Pensook, Piyanuch Anuwan, Napatsorn Kraivisitkul, Chatchadaporn Pinthong, Jittima Phonbuppha, Thikumporn Luanloet, Hein J. Wijma, Marco W. Fraaije, Narin Lawan, Pimchai Chaiyen, Thanyaporn Wongnate

https://doi.org/10.1002/cbic.201900737

Abstract

We have employed computational approaches—FireProt and FRESCO—to predict thermostable variants of the reductase component (C₁) of (4‐hydroxyphenyl)acetate 3‐hydroxylase. With the additional aid of experimental results, two C₁ variants, A166L and A58P, were identified as thermotolerant enzymes, with thermostability improvements of 2.6–5.6 °C and increased catalytic efficiency of 2‐ to 3.5‐fold. After heat treatment at 45 °C, both of the thermostable C₁ variants remain active and generate reduced flavin mononucleotide (FMNH⁻) for reactions catalyzed by bacterial luciferase and by the monooxygenase C₂ more efficiently than the wild type (WT). In addition to thermotolerance, the A166L and A58P variants also exhibited solvent tolerance. Molecular dynamics (MD) simulations (6 ns) at 300–500 K indicated that mutation of A166 to L and of A58 to P resulted in structural changes with increased stabilization of hydrophobic interactions, and thus in improved thermostability. Our findings demonstrated that improvements in the thermostability of C₁ enzyme can lead to broad‐spectrum uses of C₁ as a redox biocatalyst for future industrial applications.

Chem Bio Chem

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Effects of hydrogen peroxide on 304 stainless steel in high temperature water

S Makjan, P Boonsri, J Channuie and K Kanjana

https://doi.org/10.1088/1742-6596/1380/1/012087

Abstract

Hydrogen peroxide (H₂O₂), an oxidizer produced by water radiolysis, is considered one of the main contributors to corrosion of the stainless steel (SS) components in the cooling system of nuclear reactors. The detailed understanding of this chemical system is however still missing. The present research aimed to study the effects of H₂O₂ on 304 SS. The surface morphology and the chemical composition of the SS specimens after experiment were examined using Scanning Electron Microscope – Energy Dispersive X-ray (SEM-EDX). The change in atomic % of Fe, Cr, Ni, and O as a function of temperature will be reported. The corrosion type and possible corrosion products will be proposed and discussed.

Journal of Physics: Conf. Series

Journal of Physics: Conference Series, Volume 1380, Siam Physics Congress 2019 (SPC2019): Physics beyond disruption society 6–7 June 2019, Songkhla, Thailand

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Anti-xanthine oxidase activity of flavone analogues from dillenia indica l. and In silico study

T. Khammee, A. Rattanapittayapron, C. Rangjaroen, A. Jaratrungtawee and M. Kuno

http://dx.doi.org/10.31788/RJC.2019.1245412

Abstract

Six bioactive substances have been isolated from the fruit extract of Dillenia indica L., including a triterpene lupeol (1), together with a mixture of b-sitosterol (2a) and stigmasterol (2b), three flavonols, keamferide (3), dillenetin (4), quercetin (5), and b-sitosterol-d-glucoside (6). The structures of all isolated compounds were characterized by analysis of their spectroscopic data and by comparison with the reported value of the known compounds. The investigation of anti-xanthine oxidase activity shows that compounds 3, 4, and 5 were suitable xanthine oxidase inhibitors, with IC50 value in the range of 0.56-1.44 mM. Moreover, a molecular docking study of a potent xanthine oxidase inhibitor was performed to find the binding mode of the inhibitors into the binding site of xanthine oxidase. Keywords: Anti-xanthine Oxidase, Flavone, Dillenia indica L., Molecular Docking.

Rasayan Journal of Chemistry

Rasayan J. Chem., 12(4), 2273-2283(2019)

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Volatile constituents, in vitro and in silico anti-hyaluronidase activity of the essential oil from Gardenia carinata Wall. ex Roxb. Flowers

https://doi.org/10.33263/BRIAC96.649654

Abstract

Fresh flowers of Gardenia carinata Wall. ex Roxb. were collected from Nonthaburi, Thailand. These plant materials were extracted by steam distillation extraction. The results showed that the percentage of essential oil from steam distillation was 0.16. The extracts obtained were subjected to gas chromatography/mass spectrometry for the identification of volatile constituents. The essential oil extracted by steam distillation was characterized by the presence of trans-geraniol (1, 19.9 %) and farnesol (2, 13.2 %) as the main component. The essential oil from flowers of G. carinata and two main components were evaluated for in vitro hyaluronidase inhibitory activity using fluorometric method and compared to a reference hyaluronidase inhibitor (6-O-palmitoylascorbic acid). The results were indicated that essential oil gave the mild inhibitory activity on hyaluronidase with IC50 of 1200.4 ± 21.1 mg/mL as opposed to 6-O- palmitoylascorbic acid (IC50 =186.1 ± 3.9 mg/mL). In the case of two main components, trans-geraniol (1) and farnesol (2) displayed moderate hyaluronidase inhibition activity with IC50 value at 535.7 ± 42.2 and 292.9 ± 23.4 mg/mL, respectively. Additionally, in silico docking study of main component studies exhibited several important interactions between 1 and 2 and hyaluronidase binding site. Above finding confirmed the anti-hyaluronidase potential of G. carinata flowers.

Biointerface Research in Applied Chemistry

9(6):4649 – 4654

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Molecular Docking as a Promising Predictive Model for Silver Nanoparticle-Mediated Inhibition of Cytochrome P450 Enzymes

Nootcharin Wasukan, Mayuso Kuno and Rawiwan Maniratanachote

https://doi.org/10.1021/acs.jcim.9b00572

Abstract

Cytochrome P450 (CYP) enzymes are responsible for oxidative metabolisms of a large number of xenobiotics. In this study, we investigated interactions of silver nanoparticles (AgNPs) and silver ion (Ag⁺) with six CYP isoforms, namely CYP1A2, CYP2C9, CYP2C19, CYP2D6, CYP2E1 and CYP3A4, within CYP specific inhibitor-binding pockets by molecular docking and quantum mechanical (QM) calculations. The docking results revealed that Ag3 cluster, not Ag⁺, interacted with key amino acids of CYP2C9, CYP2C19, and CYP2D6 within a distance of about 3 Å. Moreover, the QM analysis confirmed that the amino acid residues of these CYP enzymes strongly interacted with Ag3 cluster, providing more insight into a mechanism of the potential inhibition of CYP enzyme activities. Interestingly, these results are consistent with previous in vitro data indicated that AgNPs inhibited activities of CYP2C and CYP2D in rat liver microsomes. It is suggested that the Ag3 cluster is a minimal unit of AgNPs for in silico modeling. In summary, we demonstrated that molecular docking, together with QM analysis, is a promising tool to predict AgNP-mediated CYP inhibition. These methods are useful for deeper understanding of reaction mechanisms and could be used for other nanomaterials.

Journal of Chemical Information and Modeling

J. Chem. Inf. Model. 2019, 59, 12, 5126–5134

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